A Matter of Speed: Realtime PCR for the rapid detection of mycoplasma contamination

摘要

Mycoplasma are very difficult to control as they lack the bacterial cell wall, vInch is the main point of attack for many antibiotics, for this reason, a complete retention with conventional cell structure sterile filters (0.2 μm pore size) is not possible. The danger is that the mycoplasma with a cell size between 0.5 and 0.8 pm could pass through the pores due to their great flexibility and capacity for deformation.