A simple and sensitive spectrophotometric method for estimation of diethylene triamine penta acetic acid (DTPA) in topical gel formulations

摘要

A simple, sensitive, economical and reproducible UV-Spectrophotometric method was developed for the estimation of DTPA in topical gel formulations. The method is based on NaFeDTPA complex formation by the reaction of DTPA with ferric chloride in 0.1N HCl. Optimum conditions for the reaction were investigated and absorbance was read at lambda;max at 272 nm. Bears law was obeyed in the range of 5mu;g/mL-50mu;g/mL with correlation of 0.9998. The detection and quantitation limits were found to be 0.8701 and 2.6366 mu;g/mL respectively. The proposed method was successfully applied for the determination of DTPA in topical gel formulations. Accuracy was examined through recovery studies. The results show that the procedure is accurate, precise and reproducible (relative standard deviation 1 ), while being simple, cost effective and less time consuming, which proves the suitability of the proposed method for the estimation of DTPA in topical gel formulations.