2-Aminophenoxazine-3-one Suppresses the Growth of Mouse Malignant Melanoma B16 Cells Transplanted into C57BL/6Cr Sic Mice

摘要

Since phenoxazine is an essential structure of actinomycin D,which exerts a strong anticancer effect,we examined the anticancer effect of 2-aminophenoxazine-3-one (Phx-3) on mouse malignant melanoma B16 cells in vitro and in vivo.Phx-3 inhibited proliferation of the B16 cells in a dose-dependent manner in vitro.We furthermore studied the in vivo effects of Phx-3 on mouse malignant melanoma B16 cells transplanted in female C57BL/6Cr Slc mice.Treatment with Phx-3 (0.5 mg/kg) completely suppressed the growth of mouse malignant melanoma B16 cells transplanted in mice as compared with the control group.Phx-3 was found to exert few adverse effects,in terms of bodyweight loss,changes in serum levels of blood biochemical parameters such as as-partate transaminase (AST),alanine transaminase (ALT),blood urea nitrogen (BUN) and creatinine,dysfunction of the liver and the kidney examined by pathological methods,piloerection and wasting,when mice were treated with a dose of 0.5 mg/kg.These results suggest that Phx-3 may be used to treat patients affected by malignant melanoma in future.