Antigenicity of Cell Wall Mannans of Candida albicans and Candida stellatoidea Cultured at High Temperatures in BACTEC Medium

摘要

The study of the antigenicity of pathogenic Candida albicans and Candida stellatoidea cells grown in BACTEC fungal medium (BFM) is useful for clinical analysis so as accurately to diagnose candidiasis.When C.albicans NIH A-207 was grown in BFM and fetal bovine serum-added BFM at the high temperatures of 36 and 40 deg C,the cell density increased,with a mixture of yeast cells,pseudohyphae,and hyphae and with full hyphal development in the cultures compared with cultivation (mostly cells in yeast form) at 27 deg C in both media.The mannans produced when cells were grown at these high temperatures were less reactive by enzyme-linked im-munosorbent assay with factor sera 4,5,and 6 in the commercially available kit 'Candida Check' than were the mannans obtained following growth at 27 deg C.Based on ~1H-nuclear magnetic resonance analysis,the mannans from cells grown at high temperatures had lost a phosphate group and a beta-1,2-linked mannopyranose unit,and had increased the number of non-reducing terminal alpha-l,3-linked mannopyranose units.We obtained similar re-sults for mannans produced by C.albicans J-1012,C.albicans NIH B-792,C.albicans JCM 9061,C.stellatoidea ATCC 20408,and C.stellatoidea ATCC 36232 strains cultivated in BFM at 36 deg C.These results suggest that both C.albicans and C.stellatoidea cells cultured at high temperatures,irrespective of the medium and shape of the cells,alter their antigenicity and chemical structure of cell wall mannans.