Noninvasive Measurement of Cerebral Blood Flow and Glucose Metabolic Rate in the Rat with High-Resolution Animal Positron Emission Tomography (PET): A Novel in Vivo Approach for Assessing Drug Action in the Brains of Small Animals

摘要

The cm'linil metabolic rule of glucose (CMRglc) and cerebral blood flow (CBF) in rats were estimated noniiivusivi'ly with a high-resolution animal positron emission tomography (PET) system that we recently developed, Quantifications of C'MKKIC and CBF were uttempted with radiotracers, 1HF-2-fluoro-2-deoxy-glucose and ls()-water, respectively. From the data obtained with PKT, the CMRglc and CBF values under prclraatment conditions! were respectively calculated as 51±14/mu>l/min/l(H)g and 61±7ml/min/10Og under pentobtirbital-anesthesia. These values were in close agreement with published data obtained with the autoradiographic technique. Moreover, un increase of 69% (mean) In CBF was detected after i.v. acetazol amide administration. The results indicate Ihnl this high-resolution animal PET scanner holds great potential for the assessment of drug-related metabolic tititl clrcultitory effects In intact "nd cxpcrirm'Msil iwiinmls.