arg3+, a new selection marker system for Schizosaccharomyces pombe: application of ura4+ as a removable integration marker

摘要

The yeast Schizosaccharomyces pombe is becoming increasingly popular as a genetically amenable model system for the study of diverse cellular processes from signal transduction pathways and cell-cycle control to genome organisation and recombination.A vast array of mutant strains has been created (1,2), encompassing many of these cellular functions, and mutagenesis procedures are well documented (3,4). The development of efficient transformation procedures for S. pombe (5) has allowed the cloning of wild-type fission yeast genes and functionally homologous genes from other Organisms by trans-complementation of a mutant phenotype.