Identification of novel simple sequence length polymorphisms (SSLPs) in mouse by interspersed repetitive element (IRE)-PCR.

摘要

Interspersed repetitive element (IRE)-PCR is a useful method for identification of novel human or mouse sequence-tagged sites (STSs) from contigs of genomic clones. IRE-PCR with mouse B1 repetitive element primers was used to generate novel, PCR-amplifiable, simple sequence length polymorphisms (SSLPs) from yeast artificial chromosome (YAC) clones containing regions of mouse chromosomes 13 and 14. 42 IRE-PCR products were cloned and sequenced from 8 YACs. Of these, 29 clones contained multiple simple sequence repeat units. PCR analysis with primers derived from unique sequences flanking the simple sequence repeat units in 7 clones showed all to be polymorphic between various mouse strains.