首页> 外文期刊>Nucleic Acids Research >THE RNASE P RNA FROM CYANOBACTERIA - SHORT TANDEMLY REPEATED REPETITIVE (STRR) SEQUENCES ARE PRESENT WITHIN THE RNASE P RNA GENE IN HETEROCYST-FORMING CYANOBACTERIA
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THE RNASE P RNA FROM CYANOBACTERIA - SHORT TANDEMLY REPEATED REPETITIVE (STRR) SEQUENCES ARE PRESENT WITHIN THE RNASE P RNA GENE IN HETEROCYST-FORMING CYANOBACTERIA

机译:蓝藻中的RNASE P RNA短重复序列重复(STRR)序列存在于杂合形成蓝藻中的RNASE P RNA基因内

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摘要

The RNase P RNA gene (rnpB) from 10 cyanobacteria has been characterized, These new RNAs, together with the previously available ones, provide a comprehensive data set of RNase P RNA from diverse cyanobacterial lineages, All heterocystous cyanobacteria, but none of the non-heterocystous strains analyzed, contain short tandemly repeated repetitive (STRR) sequences that increase the length of helix P12, Site-directed mutagenesis experiments indicate that the STRR sequences are not required for catalytic activity in vitro, STRR sequences seem to have recently and independently invaded the RNase P RNA genes in heterocyst-forming cyanobacteria because closely related strains contain unrelated STRR sequences. Most cyanobacteria RNase P RNAs lack the sequence GGU in the loop connecting helices P15 and P16 that has been established to interact with the 3'-end CCA in precursor tRNA substrates in other bacteria, This character is shared with plastid RNase P RNA, Helix P6 is longer than usual in most cyanobacteria as well as in plastid RNase P RNA.
机译:已经鉴定了来自10个蓝细菌的RNase P RNA基因(rnpB),这些新的RNA与以前可用的RNA一起,提供了来自各种蓝细菌谱系,所有杂性蓝细菌的RNase P RNA的全面数据集。分析的杂种菌株包含短的串联重复重复序列(STRR),可增加螺旋P12的长度。定点诱变实验表明,STRR序列不是体外催化活性所必需的,STRR序列似乎是最近独立入侵的。 RNase P RNA基因在形成异囊的蓝细菌中,因为密切相关的菌株包含无关的STRR序列。大多数蓝细菌RNase P RNA在连接螺旋P15和P16的环中缺少序列GGU,该螺旋已被确定与其他细菌的前体tRNA底物中的3'末端CCA相互作用,该特性与质体RNase P RNA,Helix P6共有在大多数蓝细菌以及质体RNase P RNA中,其长度比通常更长。

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