Affinity Chromatography. Methods and Protocols (2nd Edn, M Zachariou (ed), in Methods in Molecular Biology, Vol. 421, J. Walker (Series ed); The Humana Press, Totowa, NJ, 2008, 343 p., $99.50)

摘要

The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. Chapter 1 is an introduction summarizing the history of affinity chromatography (AC), advantages and limitation of this method, and also providing perspectives for the future development of AC techniques. Part I (chapters 1-6) highlights various modes of AC including immobilized metal ion AC of native proteins, affinity precipitation of proteins using metal chelates, immuno-AC, and dye ligand chromatography.