A novel sandwich-type electrochemical aptasensor for sensitive detection of kanamycin based on GR-PANI and PAMAM-Au nanocomposites

摘要

In this work, we report a novel sandwich-type electrochemical aptasensor for sensitive and selective detection of antibiotic kanamycin. This aptasensor was constructed by assembling graphene-polyaniline (GR-PANI) and polyamidoamine dendrimer-Au nanoparticle (PAMAM-Au) nanocomposites on the surfaces of a glass carbon electrode, which as electrode materials exhibited excellent charge-transport property and loading capacity of biomolecules. Followed by the sandwich-type specific identification, kanamycin detection was realized by outputting a redox current from electro-reduction reaction of H2O2 catalyzed by HRP. it was demonstrated that the developed strategy had a wide linear response range from 5 x 10~(-6) to 4 x 10~(-2) μg mL~(-1) and a low detection limit of 4.6 x 10~(-6) μg mL~(-1) Additionally, this aptasensor had the advantages in its low cost high sensitivity, and superb specificity. Thus, it might provide a useful tool for kanamycin determination and related food safety analysis and clinical diagnosis.