Callus induction and plant regeneration from somatic tissue in spring rapeseed (Brassica napus L.)

摘要

The effect of culture media, explants and genotypes on adventitious shoot regeneration in springrapeseed (Brassica napus L.) was examined. Hypocotyls and cotyledons of the doubled haploidlines NL-611, NL-662, NL-685 were induced to form callus by culturing on the Murashige-Skoog medium supplemented with different concentrations of 6-benzylaminopurine (BAP) anda-naphtylacetic acid (NAA) or zeatin and 2,4-dichiorphenoxyacetic acid (2,4-D). Adventitiousbuds were regenerated from the organogenic callus on the same medium. A large variation ofshoot regenerability was observed, ranging within 0-37.5% for the frequency of bud formationand within 0-3.8 for the number of buds per explant. Generally, cotyledon-derived callus ex-hibited a higher bud regeneration frequency than hypocotyls, however, hypocotyl-derived callusdeveloped a higher number of buds per explant. The maximum number of adventitious budsfrom hypocotyl-derived callus was obtained on a medium supplemented with 4.0 mg 1-' BAPand 0.05 mg 1-' NAA. Regenerated shoots were rooted in MS medium containing 0.1 mg l-1NAA. Well rooted plantlets were acclimatized and subsequently established in soil.