Immunoreactivity of orexin-containing business in the hypothalamus and the level of expression of the preproorexin gene in these cells after administration of lipopolysaccharide

摘要

The level of expression of the preproorexin gene was studied in rat hypothalamus cells 2, 4, and 6 h after administration of lipopolysaccharide (LPS) at doses of 25 and 500 μg/kg. The level of gene expression in rat hypothalamus cells 2 h after administration of both small and large doses of LPS increased, which is indirect evidence for an increase in orexin synthesis in neurons. No changes were seen at 4 and 6 h. Comparative analysis of the results of previous immunohistochemical studies and the data presented here allow the mechanisms of the reactions of orexin-containing neurons to different doses of LPS to be assessed: there was a predominance of orexin utilization processes over synthesis processes in hypothalamus cells after administration of subseptic (500 μg/kg) doses of LPS.