MULTIPLE SHOOT REGENERATION AND INDIRECT ORGANOGENESIS IN CHILLI PEPPER (Capsicum annuum L).

摘要

Repeatable protocols for rapid multiplication of a popular cultrvai of Capsicum annuum L. cv.'jwalasakhi' have been established through shoot multiplication and de novo organogenesis, Shoot tips cultured on Murashige and Skoog's (MS 1962) medium supplemented with 1-4 mg/1 BA alone or in combination with lmg/l 1AA/lBA/Kn induced multiple shoots, Maximum shoot multiplication was recorded in MS medium (MS 1962) supplemented with 4 mg/1 BA along with 2mg/l Kn. Shoot buds sprouted directly from hypocotyland cotyledon segments on MS medium containing high concentration of BA alone or in combination with 1AA/1BA or BA along with GA3 Callus initiated from cotyledonary leaf segments in a 2 mg/1 NAA containing medium upon subcultured to a 3 mg/1 BA and 1 mg/l GA3 enriched medium produced 4-6 shoot buds. Shoots developed were rooted in MS medium containing lmg/l1BA were hardened and successfully established in natural soil.