Identification of a Novel Kisspeptin with High Gonadotrophin Stimulatory Activity in the Dog

摘要

Kisspeptin (KISS1) and its receptor (KISSI r) are essential for normal reproductive function in many species, but the role of kiss1/kiss1r signalling in the dog has not yet been elucidated. The aims of this study were to identify the canine kiss 1 and kiss1r genes and to determine gonadotrophin and oes-tradiol stimulatory activity of KP-10, the shortest biologically active form of KISSI. Canine kissi and kiss1r genes were localized by comparing the reference dog genome with relevant human cDNA sequences, using BLASTn software. The amino acid sequence of canine KP-10 (YNWNVFGLRY) differs at two positions from human KP-10 (YNWNSFGLRF). A single bolus of canine KP-10 was administered intravenously to an-oestrous Beagle bitches in dosages of 0,0.1,0.2,0.3,0.5,1,5, 10, and 30 ug/kg. Blood samples were collected before and after canine KP-10 administration for the measurement of plasma luteinizing hormone (LH, all doses), follicle-stimulat-ing hormone (FSH) and oestradiol (1-30 ug/kg). From 0.2 ug/ kg onwards, canine KP-10 resulted in a rapid and robust rise in plasma LH concentration (max. at 10 min). KP-10 also resulted in a rapid and robust rise in plasma FSH concentration (max. at 10-20 min). Plasma oestradiol concentration increased significantly after dosages of 1, 5, and 10 mug/kg and reached a maximum at 60-90 min. In conclusion, canine KP-10 is a potent kisspeptin which elicits robust gonadotrophin and oestradiol responses in anoestrous bitches, suggesting that canine kissr/kiss1r are cogent targets for modulatingreproduction in dogs. E-Mail c.h.j.albers-wolthersfa'uu.nl