Effect of cytokinin 4PU30 on organ differentiation and dihydroisocoumarin production in amacha callus cultures.

摘要

Effect of a suitable combination of 1-naphthaleneacetic acid (NAA) and cytokinin N-(2-chloro-4-pyridyl)-N'-phenylurea (4PU30) concentrations, regarding organ differentiation and dihydroisocoumarin production, was examined in Hydrangea macrophylla var. thunbergii callus cultures. The callus cultured in a Linsmaier-Skoog medium containing 10(-5) M NAA and 10(-5) M 4PU30 under a light condition gave the highest level for callus growth, shoot formation, and phyllodulcin 8-O-glucoside content.