DNA interactions and promotion in antibacterial activities of the norfloxacin drug due to formation of mixed-ligand copper(II) complexes

摘要

Novel neutral mononuclear copper(II) complexes with the antibacterial drug norfloxacin in the presence of nitrogen donor heterocyclic (bipyridines) ligands were synthesized and characterized by elemental analysis, magnetic moment measurement, thermal analysis (TG), IR, LC-MS, and electronic spectra. The complexes were tested against selective gram-positive and -negative organisms for antibacterial activity. DNA binding studies were carried out using viscosity measurement and absorption titration. A DNA cleavage experiment was performed using supercoiled double-stranded DNA plas-mids in order to compare the cleavage efficiency of synthesized copper(II) complexes in the different ligand environments. Superoxide dismutase mimetic behavior was actively sought for clinical and mechanistic purposes under a nonenzymatic system and was found to have good antioxidant activity. The in vitro cytotoxic properties of the synthesized complexes were analyzed analogous to the brine shrimp bioassay.