MS-MS Fragmentation Patterns of Cholesterol Oxidation Products

摘要

This study was aimed at analyzing the daughter ion spectra of 7 toxicologically relevant cholesterol oxidation products (COPs) i.e. cholestanetriol (cholestane-3beta,5alpha,6beta-triol), 7-ketocholesterol (cholest-5-en-3beta-ol-7-one), 7alpha-hydroxycholesterol (cholest-5-en-3beta,7alpha-diol), 7beta-hydroxy-cholesterol (cholest-5-en-3beta,7beta-diol), 25-hydroxycholesterol (cholest-5-en-3beta,25-diol), alpha-epoxycholesterol (cholestane-5alpha,6alpha-epoxy-3beta-ol) and beta-epoxycholesterol (cholestane-5beta,6beta-epoxy-3beta-ol). In addition, 19-hydroxycholesterol (cholest-5-en-3beta,19-diol) was analyzed as this serves as internal standard in COPs determination by HPLC-MS.Mass spectrometry was performed using a triple quadrupol mass spectrometer that was equipped with an APCI ion source.