Ultrasensitive chemiluminescence detection of DNA on a microfluidic paper-based analytical device

摘要

A novel microfluidic paper-based chemiluminescence (CL) analytical device (μPAD) with high-throughput, rapid, stable, reusable point-of-care (POC) testing and quantitative response for DNA was designed. Sodium periodate was used to modify μPAD to covalently immobilize capture DNA. The paper device was fabricated by a low-cost, simple, and rapid wax screen-printing method. The developed paper-based CL immunodevice, combined with a typical luminol-H2O2 CL system, showed excellent analytical performance for the detection of DNA. Moreover, gold nanoparticles for signal DNA immobilization were used for signal-on CL assay of target DNA, which greatly improved the sensitivity. This study shows the successful integration of the μPAD and the CL method to afford an easy-to-use, inexpensive, and portable alternative for POC monitoring.