Destruction of Cucumber Green Mottle Mosaic Virus by Heat Treatment and Rapid Detection of Virus Inactivation by RT-PCR

摘要

Heat treatment is commonly used to control viral con-tamination of seeds. To study virus inactivation, virus was purified from seeds contaminated with Cucumber green mottle mosaic virus (CGMMV) after various heat treatments. CGMMV particles were observed to be physically disrupted by high temperature. Analysis of viral RNA revealed that the 5' and 3' termini of the genome were protected whereas regions between 2-2.5, 3.2-3.7 and 4-4.8 kb from the 5' terminus were not. Heat inactivation of virus on seeds was confirmed by RT-PCR using primers for a heat-sensitive region. The RT-PCR approach developed here may prove prefer-able to time- and labor-intensive bioassays for assess-ing virus heat inactivation.