The Pap1-independent Inducation by Metal Ions of a Third Gene Encoding Glutathione S-Transferase Gene from the Fission Yeast

摘要

A third gene that encodes glutathione S-transferase (GSTIII) was previously cloned from the fission yeast Schizosaccharomyces pombe. Using the GSTIII-lacZ fusion plasmid pGDA-19, its expression was shown to be enhanced by various metal ions. In the present study, four additional fusion plasmids, pGDA-29, pGDA-39, pGDA-49, and pGDA-59, were designed to carry 998, 378, 276, and 115 bp upstream regions from the translational initiation point, respectively. The major activation region was located between -998 and -378 bp upstream of the GSTIII gene. Regulatory sequences that are responsible for the induction by metal ions reside between -998 and -378 bp and between -276 and -115 bp upstream of the gene. The overexpressed Pap1 exerts a repression effect on the GSTIII expression via -998 ～ -378 bp region, whereas it exerts an activation effect on the GSTIII expression via -270 ～ -115 bp region. However, the induction of the GSTIII gene by metal ions occurs independent of Pap1.