Gossypium raimondii: a source of fertility restorer for cytoplasmic male sterility of Gossypium hirsutum L.

摘要

Comparative studies of pollen fertility restoration in different triploid hybrids using cytoplasmic male sterile (CMS) line of Gossypium hirsutum 76 IH (2n = 4x = 52; A(h)A(h)D(h)D(h)),wild species and perennial cottons were studied. Triploid hybrid (3n = 39; A(h)D(h)D(5)) involving G. raimondii (2n = 26; D-5 D-5) was vigorous growing and partially fertile. Restoration of pollen fertility observed in this hybrid was attributed to bivalent formation (11.13(II)/PMC) leading to formation of 40% fertile pollen. In contrast to the CMS female parent, arrestation of meiosis during early prophase and degeneration of PMCs was not observed in triploid hybrid that indicated existence of fertility restorer factor in G. raimondii. Efforts were made to induce chromosome doubling as well as induce boll setting in this hybrid with the help of different treatments of colchicine and growth hormones, respectively. The fertility restorer factor reported in present report will be an alternative source of restorer gene in G. hirsutum L. cotton added with tolerance to sucking pests, drought and desired fibre properties. [References: 27]