Main biological determinants of Continuous Cell Lines elucidated by a principal component analysis

摘要

Cytomorphological, morphometric, quantitative cytochemical and population indices of ten con-tinuous cell lines of different origin were studied in the course of cultivation. Principal component analysis and hierarchical clustering identified two clusters of cell lines characterized by different behavior during cultivation. Cell lines in first cluster (NIH 3T3, SHSY 5Y, CaCo-2, BHK-21, RD) show mainly low transcriptional activity, stabilization of main indices at the early time of the cultivation, and higher number of cells per unit area; where-as second cluster (Hep-G2, HEK 293, Hep3B, HEp-2, Hela) characterized by high transcriptional activity, ac-tive growing population which after a growth phase undergoes degeneration of cellular culture or stabilization on low level of transcriptional activity. In conclusion, we showed that the cell line behavior during cultivation positively correlate with factors related to transcriptional activity of cells (nucleolar indices, RNA content, etc) and negatively with euploidy and cell number in confluence.