Optimization of microwave-assisted extraction of resveratrol from polygonum cuspidatum sieb et Zucc by orthogonal experiment

摘要

In order to optimize the conditions for the microwave-assisted extraction of resveratrol from Polygonum cuspidatum Sieb et Zucc, in basis of microwave-assisted extraction time,solvent concentration , ratio of liquid to solid, microwave power four single factor test, the optimum conditions for the microwave-assisted extraction of resveratrol from Polygonum cuspidatum Sieb et Zucc are determined by orthogonal experiment. The optimum extraction conditions confirmed by the index of extraction yield were as follows : microwave-assisted extraction time 7 min, solvent concentration 80% (v/v)ethanol, ratio of liquid to solid 25:1 (ml:g),microwave power 1.5 kw. The extraction yield of resveratrol was 1.76%. Mass spectrum analysis showed that extract was resveratrol.