Isoation, Identification and Sequencing of the He-Pro Gene of Papaya Ringspot Virus (PRSV-P)

摘要

The study was taken up to isolate, identify and sequence the He-Pro gene from a papaya ringspot virus (PRSV) strain infecting papaya. The total RNA was extracted from infected papaya leaf and the He-Pro gene specific primer designed in-silco and the cDNA synthesized, PCR was performed to amplify the gene which was checked and the amplicon was eluted and ligated into pTZ57R/T vector. The clone was confirmed using blue white colony screening and colony PCR. Positive cloned plasmid was sequenced and analyzed. Nucleotide BLAST analysis shows that homology between cloned sequence with WB-1 He-Pro was 97 per cent.