Two hundred and fifty grams of Grains viz., Ragi, Sorghum, Maize, Paddy (ill filled grains) and Wheat (ill filled grains) grains are pre-soaked in water for overnight in polypropylene bags and water was decanted thoroughly. Manures and other substrates (Table I) were also taken in a polypropylene bags @ 250gm / bag and are moistened with sterile water. All the substrates were sterilized at 121°C for one hour for two consecutive days and inoculated with spore suspension (6 x 10’spores/ml) of T. viride @ 1 ml/bag. Each treatment was replicated thrice and inocubated at room temperature (28 ± 2°C) for 15 days. The sample (4g) was drawn on 15 days after inoculation and colony forming units were enumerated on Trichoderma specific media (Elad and Chet. 1983) using dilution plate technique and data analyzed statistically.
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机译：将250克谷物，拉吉（Ragi），高粱，玉米，稻田（不良填充谷物）和小麦（不良填充谷物）谷物预先浸入水中，并在聚丙烯袋中浸泡过夜，然后将水彻底倒出。粪便和其他底物（表I）也装在250gm /袋的聚丙烯袋中，并用无菌水润湿。将所有底物在121°C下连续两天灭菌一小时，并用1 ml /袋的六面体三叶草的孢子悬浮液（6 x 10'spores / ml）接种。每个处理重复三次，并在室温（28±2°C）下孵育15天。接种后15天抽取样品（4g），并使用稀释平板技术在木霉属特异性培养基（Elad and Chet。1983）上计数菌落形成单位，并进行统计分析。
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