Characterization of Triterpenoids, Flavonoids and Phenolic Acidsin Ecliptaprostrata by High-performance Liquid Chromatography/diode-arrayDetector/electrospray lonization with Multi-stage Tandem Mass Spectroscopy

摘要

High-performance liquid chromatographic method with diode-array detector and electrospray ionizationwith multi-stage tandem mass spectroscopy (HPLC/DAD/ESI-MS~n) was used to identity the major constituents ina methanolic extract of Eclipta prostrata. The chromatographic separation was performed on a C18 column.Acetonitrile-water was used as a mobile phase. HPLC/DAD/ESI-MS~n allowed the characterization of constituentsof E. prostrata, mainly triterpenoids (eclalbasaponin I, II, in, IV, VI), flavonoids (luteolin 7-O-glucoside,demethylwedelolactone, wedelolactone, luteolin, demetylwedelolactone sulfate, luteolin sulfate, apigenin sulfate)and phenolic acids (5-O-caffeoylquinic acid, 3, 4-O-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, 4, 5-0-dicaffeoylquinic acid).