The Isolation of Acetylcholinesterase Inhibitory Constituents from Lycoris radiata using On-line HPLC-biochemical Detection System

摘要

Bioactivity-guided fractionation using on-line HPLC biochemical detection system on CHCl_3-soluble fraction of Lycoris radiata led to the isolation of deoxylycorenine (1), O-demethylhomolycorine (2), galanthamine (3), lycoramine (4), mixture of 6a-and 6P-haemanthidine (5), and lycorine (6), identified by spectroscopic data and physicochemical property. Among the isolated compounds, 1,3 and 6 showed acetylcholinesterase inhibitiory activities with IC_(50) values of 18.0, 12.0 and 16.6 muM, respectively, in in vitro colorimetric microplate assay.