A multi-omic map of the lipid-producingyeast Rhodosporidium toruloides

摘要

Triacylglycerols are among the most attractive alternative raw materials for biofueldevelopment. Current oil plant-based technologies are limited in terms of triacylglycerolproduction capacity and rate. These limitations may be circumvented by biotransformation ofcarbohydrates into lipids; however, our understanding of microbial oleaginicity remains limited.Here we present the results of a multi-omic analysis of Rhodosporidium toruloides, a robusttriacylglycerol-producing fungus. The assembly of genome and transcriptome sequencingdata reveals a genome of 20.2 mb containing 8,171 protein-coding genes, the majority of whichhave multiple introns. Genes including a novel fatty acid synthase are predicted to participatein metabolic pathways absent in non-oleaginous yeasts. Transcriptomic and proteomic datasuggest that lipid accumulation under nitrogen-limited conditions correlates with the inductionof lipogenesis, nitrogenous compound recycling, macromolecule metabolism and autophagy.The multi-omic map of R. toruloides therefore provides a valuable resource for efforts torationally engineer lipid-production pathways.