[I-125]-ATRIAL NATRIURETIC PEPTIDE BINDING SITES IN THE AMPHIBIAN TESTIS

摘要

Atrial natriuretic peptide-like immunoreactivity (XNP-like IR) was shown in the tests of Bufo marinus, in the head of mature spermatozoa attached to Sertoli cells, interstitial tissue and seminiferous tubules. After spermiation induced by human chorionic gonadotropin (HCG), testicular spermatozoa failed to show IR. ANP binding sites were localized and characterized in testis of Bufo murinus by quantitative in vitro autoradiography using [I-125]-ratANP(99-126). Specific ANP binding demonstrated positive cooper-activity. The data suggest a new biological function of ANP in reproduction of male amphibians.