QPrEST (TM)-isotope-labeled multipeptide standards for quantitative mass spectrometry-based proteomics

摘要

Mass spectrometry (MS) enables absolute quantification of endogenous proteins by the use of isotope-labeled standards as internal references. QPrEST standards, currently available for >13,000 human proteins, represent a novel class of recombinantly produced heavy isotope-labeled standards that are added early in the quantification workflow. These multipeptide standards contain 50-150 amino acids identical to a human target sequence, resulting in absolute quantification data based on multiple tryptic peptides.