Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately,most probes in this class are hampered by oligomerization, small photon budgets or poor photostability.Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations,exhibits high photostability and preserves the similar to 10-nm localization precision of its parent.
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