Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging.

摘要

There was no road map for how to do this. But Joe Gall had already shown that a fraction of the molecules were circular when extracted from cells, a property reminiscent of-phage lambda, the linear DNA of which circularizes to replicate. Because Liz had grown to believe that nature uses elegant and universal solutions, she thought that the lambda ends might be a possible model for the molecular nature of the Tetrahymena termini. Thus, she decided to use in vitro the DNA'repair' reaction of DNA polymerase, which Jiad been successfully used by Ray Wu and colleagues to sequence the cohesive ends of the lambda phage family genomic DNAs7.