Poly D, L-lactide-co-glycolic acid (PLGA) entrapped ehitin on innate and adaptive immune responses in Epinephelus bruneus against Vibrio alginolyticus

摘要

The efficacy of poly D, L-lactide-co-glycolic acid (PLGA)-entrapped ehitin (CI) on innate and adaptive immune response in kelp grouper {Epinephelus bruneus) against Vibrio alginolyticus was investigated at weeks 1, 2, and 4. The myeloperoxidase (MPO) activity significantly increased in ehitin (CI) and PLGA-entrapped ehitin (PLGA+CI) on week 4. The serum haemoagglutinating activity and haemolysin activity were significantly enhanced in CT and PLGA+CI on weeks 2 and 4. The respiratory burst (RB) activity and bacterial agglutination activity were increased significantly in ehitin (CI) and PLGA-entrapped ehitin (PLGA+CI) from weeks 1 to 4. The cumulative mortality was found low in PLGA-entrapped ehitin with 15% whereas 30% and 25% in PLGA and ehitin. The present results suggest that PLGA-entrapped ehitin microsphere act as immunostimulant that enhance the innate and adaptive immune system in kelp grouper against V. alginolyticus infection.