The Study of Estimation of Chromatin Abnormality of Ogye Rooster Sperm and Activity by Diff-Quik Staining Method

摘要

Ogye rooster sperm chromatin status can be detected using well-established sperm assays. In this paper, a simple and fast method for monitoring rooster sperm chromatin status was employed in the field for the assessment of chicken sperm quality. Usingstandard bright field microscopes, Diff-Quik stains can be reproducibly, easily and routinely monitored with simple staining. The presence of abnormal chromatin staining of the rooster sperm was determined based on the presence of darker staining in thehead. In the fresh semen, the viabilities of the spermatozoa from three tested Ogye were 93.53%, 82.42% and 90.63%, and normal chromatin rates were 87.96%, 74.25% and 85.10%, respectively. However, after freezing, the viability rates of the thawed semenwere reduced to 69.58%, 61.98% and 72.20%, and the normal chromatin rates were also reduced to 58.91 %, 48.49% and 63.34%. The significant correlations between the live sperm and the normal sperm nuclei were 0.875 in the fresh semen and 0.513 in the frozen semen. After the incubation of the sperm at 37°C for 5 min, the rates of viability, chromatin normality and sperm head activity were found to be 90.63±1.28%, 82.44±8.09% and 66.68± 10.29%, respectively, in fresh semen. However, the correspondingrates in the thawed semen were reduced to 67.92±7.55%, 56.92±12.15% and 47.32 ±5.02%. The relationship between chromatin normality and sperm head movement in the fresh and thawed semen were 0.564 and 0.540, respectively. These results indicate that chicken sperm normality can be assessed with Diff-Quik staining, which could be used as a simple and rapid staining method for assessing the chromatin status of sperm heads and the activated morphologies of live spermatozoa.