Preparation of Functional Cosmetics Containing p-carotene Derived from Recombinant Escherichia coli and Evaluation of Anti-wrinkle Efficacy by Clinical Testing

摘要

This paper described the formulation of functional cosmetics and evaluation of anti-wrinkle efficacy in clinical test. Cosmetics were formulated with highly purified beta-carotene obtained from the culture broth of recombinant E.coli cells. Edible oilfor solubilizing beta-carotene, vitamine E for long-term storage, detergent/stabilizer (2.0%) for the complete formation of oil/water emulsion, dimethicone (0.35%) for good skin care, and sorbitol for skin moisturizer were also added as ingredients. Physical or chemical degradation of formulated products stored at 0 deg C, 25 deg C, or 40 deg C was not observed for 60-day testing period. In clinical test, 68% of applicants observed wrinkle decrease after 8-week treatment. This result indicates that newly formulated cosmetics have strong potential for improving wrinkle skin care.