Toxicity Evaluation of Organochloride Pesticide, Endosulfan and its Metabolites Using Microalgae

摘要

A growth inhibition assay using Chlorella sp. AG 10002 based on the OECD 201 standard test procedure was applied to the toxic-ity testing of endosulfan and its reported metabolites. Comparison of dry cell weight, optical density (OD) at 680 nm, and chlorophyll a concentration indicated that optical density at 680 nm of culture broth is convenient, rapid, and accurate method for cell growth. In this microalgae system, the IC_(50) values of endosulfan, endosulfan sulfate, endosulfan lactone, and endosulfan ether were determined as 9.45, 18.8, 18.2 and 37.5 mg/ L, respectively. In a while, endosulfan diol did not show a significant toxicity up to 50 mg/L. Since eridosulfan is liable at acidic or alkaline conditions, treatment of endosulfan in pH 3, 4,and 11 for 3 days resulted in reduced toxicity, as expected. These results suggested that the microalgae system is useful to evaluate various toxic chemicals and provide a new notion for bioremediation of endosulfan in aqueous systems.