Protective Effects of Cellular Membrane and Component Analysis of Polygonum aviculare Extracts

摘要

In this study, the antioxidative effects and component analysis of Polygonum aviculare (P. aviculare) extracts were investigated. The ethyl acetate and the aglycone fraction from P. aviculare extracts were more active than (+)-alpha-tocopherol and L-ascorbic acid, which are known as strong antioxidants for their antioxidative activity by the DPPH method and chemiluminescence assay. The cellular protective effects of fractions of P. aviculare on the rose-bengal sensitized photohemolysis of human erythrocytes, increased in a concentration dependent manner (1-10 mul). In particular, the ethyl acetate fraction at a concentration of 10 mul showed the most prominent protective effect among all the extracts (tau_(50), 314.70 min). TLC and HPLC chromatogramof the ethyl acetate fraction of P. aviculare extracts revealed 3 main bands (PA8, PA5, PA6) and peaks (peak 1, peak 2, peak 3), which were identified as myricetin-3-omicron-rhamnoside (myricitrin, PA8, peak 1), quercetin-3-alpha-rhamnoside (quercitrin,PA6, peak 3) by LC/ESI-MS/MS and ~1H-NMR respectively. These results indicate that fractions from P. aviculare could be applicable to new functional cosmetics as antioxidants.