Protective Effect of Radiation-induced New Blackberry Mutant gamma-B201 on H_2O_2-induced Oxidative Damage in HepG2 Cells

摘要

The objective of the present study was to investigate the chemical composition of anthocyanin-enriched extract of radiation-induced blackberry (Rubus fruticosus L.) mutant (gamma-B201) as well as the protective effect of gamma-B201 against oxidative stress in vitro. The cytotoxicity, reactive oxygen species (ROS) scavenging capacity, and DNA damage were assessed by WST-1 assay, flow cytometry, and comet assay, respectively. Lactate dehydrogenase, superoxide dismutase, and catalase activities were determined by using a commercial kit. The in vitro results showed that gamma-B201 increased the cell viability, reduction of lactate dehydrogenase release, and intracellular ROS scavenging capacity in hydrogen peroxide (H_2O_2)-treated HepG2 cells. Furthermore, treatment with gamma-B201 attenuated DNA damage in H_2O_2-treated HepG2 cells and treatment with gamma-B201 restored the activity of superoxide dismutase and catalase in H_2O_2-treated HepG2 cells. In conclusion, the present study suggests that gamma-B201 blackberry extract can exert a significant cytoprotective effect against H_2O_2-induced cell damage.