Effects of persimmon leaf tea extract, green tea extract and Oolong tea extract on the frequencies of mutagen-induced sister chromatid exchange in Chinese hamster ovary cells

摘要

The suppressing effects of crude extracts of three Korean teas, persimmon leaf tea extract (PLTE), green tea extract (GTE) and oolong tea extract (OTE), were studied on the induction of sister chromafid exchange (SCE) in cultured Chinese hamster ovary cells. When cells were heated with tea extract after mitomycin C (MMC) treatment, the frequency of MMC-induced SCEs were decreased at the high concentration (1000 #mu#g/mL) of PLTE in the presence of S9 mix and at low concentrations (20 approx 80 #mu#g/mL) of PLTE in the absence of S9 mix. Whereas GTE and OTE showed suppressing effects on the MMC-induced SCEs at low concentrations (10 approx 20 #mu#g/mL) for OTE and 160 #mu#g/ mL for GTE only in the presence of S9 mix. MMC-induced SCEs were decreased by post-treatment with each tea extracts with S9 mix in the G1 phase of the cell cycle. These results suggest that PLTE, GTE and OTE could have bio antimutagenic activities, and also suggest that PLTE might have unknown antimutagenic components which would be responsible for the inhibitory effect against direct acting mutagenicity.