Genetic diversity and strain-typing in cultivated strains of Lentinula edodes (the shii-take mushroom) in Japan by AFLP analysis

摘要

Amplified fragment length polymorphism (AFLP) analysis is based on selective PCR (polymerase chain reaction) amplification of genomic DNA restriction fragments. The present study was performed to: (11) assess the extent of AFLP variation among major cultivated strains of Lentinula edodes (the shii-take mushroom) in Japan; (2) evaluate the usefulness of AFLP as genetic marker for typing the cultivated strains, and (3) infer the genetic relatedness among them. Six AFLP primer pairs detected a total of 304 DNA fragments in a sample of 13 cultivated strains for wood log cultivation and 2 strains for sawdust cultivation, of which 179 DNA fragments (58.9 % of detected fragments) were polymorphic between two or more strains. These polymorphic DNA fragments could differentiate all of the cultivated strains. Cluster analysis and principle coordinate analysis based on AFLP data showed two distinct groups; one group was composed of the strains in which fruiting occurred during the middle to low temperature period from autumn to next spring under outdoor wood log cultivation, and the other strains in which fruiting could be induced in the summer under outdoor cultivation and strains for sawdust cultivation under indoor air-controlled condition.