Stress response of Nidula niveo-tomentosa to UV-A light

摘要

Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomeutosa. To investigate the Fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione s-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.