Colorimetric method for determining hydrogen peroxide production in liquid media by filamentous fungi

摘要

A semiquantitative assay to estimate rates of H2O2 produced in the liquid medium by fungi grown in a modified Georgiou-Petri dish growth apparatus in 9-cm Petri dishes is described. The method is based on the horseradish peroxidase-mediated oxidation of phenol red by H2O2 adapted to assay nmol amounts of H2O2 produced by fungar mycelial colonies in their own liquid media. We showed for the first time that the phytopathogen basidiomycete Sclerotium rolfsii produces H2O2 in the growth medium with the highest production rate of 250 nmol/min/ g fungal dry weight at 3 d and with the highest accumulation rate of 235 nM/min at 8 d. The assay is simple and when proper ly adjusted can provide a reasonable in vitro assessment of the capacity of various fungi to produce H2O2 providing a valuable tool for studying its involvement in fungal phytopathogenicity and in microbial oxidative stress. [References: 18]