A morphologically distinct strain of Aspergillus nomius

摘要

DNA polymorphisms were used to infer relationships of a morphologically distinct new strain of aflatoxin-producing Aspergillus to other members of Aspergillus sect. Flavi. The new strain produced both B and G aflatoxins, and differed both morphologically and physiologically from other aflatoxin producers. Three isolates of the new fungus were compared with representatives of 3 known aflatoxin-producing species. Regions of the Taka-amylase A, beta-tubulin, calmodulin, and nitrate reductase genes were amplified using PCR and digested with restriction enzymes. RFLP analysis of all 4 gene regions suggested that the new Aspergillus isolates are closely related to A. nomius, but are diverged from other aflatoxin-producing species.