Chromoblastomycosis due to Fonsecaea pedrosoi and F. monophora in Cuba. (Special Issue: The bright future of darkness - the rising power of black fungi: black yeasts, microcolonial fungi, and their relatives.)

摘要

We report two cases of chromoblastomycosis due to Fonsecaea pedrosoi and F. monophora in otherwise healthy Cuban males. Direct microscopic examination of biopsies revealed muriform cells, the hallmark of chromoblastomycosis. The suspected agents were recovered in culture, identified on the basis of morphological criteria and confirmed by sequencing of the internal transcribed spacer regions of rDNA. Final treatment consisted of surgical excision. The patients were successfully cured since there was no relapse after a follow-up of more than a year. In vitro antifungal susceptibility testing of both isolates showed that itraconazole and posaconazole had potent activity. High MICs of amphotericin B (2 micro g/ml), fluconazole (>64 micro g/ml), anidulafungin (8 micro g/ml) and caspofungin (8 micro g/ml) were found.