Establishment of an in Vitro Peyer's Patch Cell Culture System Correlativeto in Vivo Study Using Intestine and Screening of Lactic Acid BacteriaEnhancing Intestinal Immunity

摘要

Some lactic acid bacteria (LAB) are known as representative of probiotics. To screen LAB effective toenhance intestinal immunity, in the present study, we developed an accurate and convenient in vitro evaluationsystem using Peyers patch cells (PP-cells) isolated from the mice intestine. We observed that the amount ofimmunoglobulin A (IgA) produced by PP-cells co-cultured with LAB was well correlative to that in PP-cells,intestine and feces isolated from live mice after oral administration of LAB [correlation coefficient (r)=0.888,0.883, and 0.920, respectively]. In addition, using this in vitro system, we suggest that the IgA level of PP-cells co-culturing with plant-derived LAB might be more enhanced than with animal-derived LAB.