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Eighteen new tetranucleotide microsatellite DNA markers for Coregonus lavaretus cloned from an alpine lake population

机译:从高山湖泊种群克隆的Coregonus lavaretus的十八个新的四核苷酸微卫星DNA标记

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摘要

We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic variation was high with nine to 37 alleles per locus and expected heterozygosities ranging from 0.37 to 0.95. The high level of polymorphism revealed by these loci will be relevant for population studies in context to the evolutionary history of this species.
机译:我们从丰富的(GACA)(n)和(GATA)(n)重复序列的基因组库中为Coregonus lavaretus开发了18个多态微卫星标记。重点放在开发高度多态的,完美的重复序列上。在来自奥地利两个高山种群的69个个体中筛选了这些基因座。等位基因变异很高,每个位点有9至37个等位基因,预期杂合度为0.37至0.95。这些基因座揭示的高水平多态性将与该物种的进化史相关的种群研究相关。

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