Development of polymorphic microsatellite from RAPD bands of black sea bream Acanthopagrus schlegeli

摘要

Eight pairs of simple sequence repeat markers were developed from random amplified polymorphic DNA product in black sea bream Acanthopagrus schlegeli. Twenty microsatellites were selected for designing microsatellite primers, of which eight gave working primer pairs. They had between three and seven alleles. Observed and expected heterozygosities varied from 0.65 to 0.90, and from 0.58 to 0.82, respectively. Eight additional fish species assessed for cross-species amplification revealed between two and five positive amplifications and between zero and three polymorphic loci per species.