Isolation and characterization of microsatellite loci in the tapeworm Ligula intestinalis (Cestoda : Pseudophyllidea)

摘要

Ligula intestinalis is a species with a complex biology. Several strains, distinct in the host specificity and biogeography, are supposed to exist; however, their morphological or molecular identification remains unresolved. We describe the isolation of 16 polymorphic microsatellite markers. The number of alleles ranged from four to 14 in a population of 21 L. intestinalis individuals. Loci showed amplification success across samples of worldwide distribution. A cross-amplification of 14 markers in Digramma interrupta (Cestoda: Pseudophyllidea) was observed.