Characterization of polymorphic microsatellite loci from black grouper, Mycteroperca bonaci (Teleostei: Serranidae)

摘要

Many groupers in the western Atlantic are vulnerable to overfishing, and appropriate management requires knowledge of the population structure of these fish. To this end microsatellite markers were developed from black grouper, Mycteroperca bonaci, using an enrichment procedure. Five loci with levels of polymorphism ranging from 0.5 to 0.9 were selected. These markers also proved to be useful in scamp, M. phenax; red grouper, Epinephelus morio; and goliath grouper, E. itajara. Conformation to Hardy-Weinberg equilibrium was observed except for heterozygote deficiencies noted for E. morio at two of the loci used for this species.