...
首页> 外文期刊>Molecular ecology notes >Development and characterization of SSR markers in Ribes species
【24h】

Development and characterization of SSR markers in Ribes species

机译:里氏菌种SSR标记的开发与鉴定

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Eleven microsatellite loci were identified and characterized in blackcurrant (Ribes nigrum L.) and related species. An enriched library was constructed and screened with simple sequence repeat (SSR) oligonucleotides. Positive clones were sequenced and primers were designed flanking the repeat motifs. These 11 microsatellites produce amplification products polymorphic across a range of Ribes germplasm, predominantly from the Eucoreosma section of the genus. The number of alleles varied from 2 to 18 with levels of diversity ranging from 0.18 to 0.91.
机译:在黑加仑(Ribes nigrum L.)和相关物种中鉴定并鉴定了11个微卫星基因座。构建丰富的文库,并用简单序列重复(SSR)寡核苷酸进行筛选。对阳性克隆进行测序,并设计重复序列两侧的引物。这11个微卫星产生的扩增产物在一定范围的Ribes种质上具有多态性,主要来自该属的Eucoreosma部分。等位基因的数量从2到18不等,多样性水平从0.18到0.91。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号